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AP Biology Unit 3 Review Practice Quiz

Tackle FRQs, MCQs, and test challenges now

Difficulty: Moderate
Grade: Grade 12
Study OutcomesCheat Sheet
Colorful paper art promoting AP Bio FRQ Face-Off trivia challenge for high school students.

Which process produces the most ATP during cellular respiration?
Electron transport chain
Glycolysis
Fermentation
Krebs Cycle
The electron transport chain produces the majority of ATP through oxidative phosphorylation. Glycolysis and the Krebs cycle contribute far less ATP compared to this process.
Which organelle is primarily responsible for ATP production in eukaryotic cells?
Endoplasmic reticulum
Ribosome
Golgi apparatus
Mitochondrion
Mitochondria are known as the powerhouse of the cell because they generate ATP through oxidative phosphorylation. Other organelles perform different cellular functions unrelated to ATP synthesis.
Which pigment is primarily responsible for capturing light energy in plants?
Carotenoid
Phycobilin
Chlorophyll
Xanthophyll
Chlorophyll is the main pigment that absorbs light energy for photosynthesis. Although other pigments can absorb light as accessory pigments, chlorophyll is crucial for energy capture.
Which process does not require oxygen?
Photosynthesis
Aerobic respiration
Fermentation
Citric acid cycle
Fermentation allows cells to produce ATP in the absence of oxygen by regenerating NAD+ for glycolysis. In contrast, aerobic respiration and the citric acid cycle rely on oxygen.
What is the primary role of enzymes in metabolic reactions?
Provide energy for reactions
Change the equilibrium
Lower the activation energy
Produce ATP exclusively
Enzymes speed up metabolic reactions by lowering the activation energy required, making the reactions proceed faster. They do not alter the equilibrium or provide energy directly.
What is the end product of glycolysis?
Pyruvate
Lactate
Acetyl-CoA
Oxaloacetate
Glycolysis converts one molecule of glucose into two molecules of pyruvate through a series of enzyme-catalyzed steps. Pyruvate is then used in subsequent metabolic pathways depending on oxygen availability.
Where does the Krebs cycle occur in eukaryotic cells?
Mitochondrial intermembrane space
Mitochondrial matrix
Cytosol
Chloroplast stroma
The Krebs cycle takes place in the mitochondrial matrix where the necessary enzymes and substrates are located. It does not occur in the cytosol or within chloroplasts.
Which component of the electron transport chain is a mobile electron carrier?
Cytochrome c
ATP synthase
NADH dehydrogenase
Succinate dehydrogenase
Cytochrome c is a mobile electron carrier that shuttles electrons between complexes in the electron transport chain. The other options are large, integral membrane proteins that do not move freely.
Which process fixes CO2 in plants?
Light-dependent reactions
Glycolysis
Krebs cycle
Calvin cycle
The Calvin cycle is responsible for incorporating CO2 into organic molecules during photosynthesis. The light-dependent reactions generate the energy-rich molecules needed for this process.
Which of the following best describes allosteric regulation in enzymes?
A change in substrate concentration altering enzyme structure
Regulation through binding at a site other than the active site
Enzyme inhibition by blocking the active site
Covalent modification always deactivates enzymes
Allosteric regulation involves the binding of molecules to sites other than the enzyme's active site, thereby changing its conformation and activity. This mechanism provides fine control over metabolic pathways.
Which fermentation pathway regenerates NAD+ by converting pyruvate into lactate?
Alcoholic fermentation
Oxidative phosphorylation
Lactic acid fermentation
Citric acid cycle
Lactic acid fermentation converts pyruvate into lactate, which regenerates NAD+ required for glycolysis to continue. Alcoholic fermentation, on the other hand, produces ethanol and CO2.
What role does ATP synthase play in cellular respiration?
It breaks down glucose in the cytosol
It oxidizes NADH in glycolysis
It synthesizes ATP using the proton gradient
It transports electrons in the electron transport chain
ATP synthase uses the energy from the proton gradient established by the electron transport chain to generate ATP. It does not transport electrons or directly oxidize NADH.
Which accessory pigment aids in protecting chlorophyll and expanding the range of light absorption?
Chlorophyll b
Phycocyanin
Carotenoids
Anthocyanin
Carotenoids serve as accessory pigments by broadening the spectrum of light absorption and protecting chlorophyll from photodamage. They play a crucial photoprotective role in photosynthesis.
Which molecule transports electrons from glycolysis to the electron transport chain?
ADP
NADH
Coenzyme A
FADH2
NADH, produced during glycolysis, carries electrons to the electron transport chain where they are used for ATP production. FADH2 is generated later during the Krebs cycle.
What effect do high levels of ATP have on phosphofructokinase during glycolysis?
They convert the enzyme into a different form
They have no effect on the enzyme
They have an inhibitory effect
They activate the enzyme
High levels of ATP act as a negative feedback inhibitor on phosphofructokinase, slowing down glycolysis when energy is abundant. This regulation helps maintain energy balance in the cell.
A mutation reduces the affinity of a glycolytic enzyme for its substrate. Which kinetic parameter is primarily affected and what is the metabolic consequence?
Increase in Vmax, causing a faster rate of glycolysis
Increase in enzyme specificity, enhancing efficiency
Increase in Km, leading to a decreased rate of glycolysis
Decrease in enzyme concentration without affecting kinetics
A lower substrate affinity is reflected by an increased Km, meaning that a higher concentration of substrate is needed to achieve half-maximal enzyme activity. This results in a slower glycolytic rate under normal conditions.
Inhibition of Complex III in the electron transport chain results in immediate accumulation of which of the following?
Direct inhibition of glycolysis
Increased ATP synthesis
Reduced cytochrome c
Enhanced oxygen uptake
Inhibiting Complex III prevents the forward flow of electrons, causing an accumulation of reduced electron carriers such as cytochrome c. This disruption impairs the overall function of the electron transport chain.
Under anaerobic conditions, how does the ATP yield per molecule of glucose via fermentation compare to that of aerobic respiration, and why?
Fermentation yields a higher ATP yield because it is a faster process
Fermentation yields lower ATP because it bypasses oxidative phosphorylation
Both processes yield the same amount of ATP
Fermentation increases ATP yield by enhancing glycolytic efficiency
Fermentation relies solely on glycolysis for ATP production, omitting the energy-rich steps of oxidative phosphorylation present in aerobic respiration. This results in a significantly lower ATP yield per glucose molecule.
What impact would a mutation affecting the D1 protein of photosystem II have on the light-dependent reactions?
No change in photochemical efficiency
Accumulation of protons in the thylakoid lumen enhancing ATP synthesis
Decreased water splitting efficiency, reducing electron flow
Increased production of NADPH
The D1 protein is essential for the proper function of photosystem II, specifically in splitting water molecules. A mutation in this protein leads to reduced water splitting and diminished electron flow, impairing the light-dependent reactions.
Why is the regeneration of NAD+ essential for the continuation of glycolysis under anaerobic conditions?
NAD+ is not needed for glycolysis under anaerobic conditions
NAD+ accumulation would inhibit lactate dehydrogenase
Excess NAD+ would slow down glycolysis due to product inhibition
Without NAD+, glycolysis would halt because glyceraldehyde-3-phosphate dehydrogenase would lack its cofactor
NAD+ is a necessary cofactor for the glyceraldehyde-3-phosphate dehydrogenase step in glycolysis. In anaerobic conditions, regenerating NAD+ via fermentation is crucial to keep glycolysis running for ATP production.
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Study Outcomes

  1. Analyze authentic free-response questions to identify key biological concepts.
  2. Interpret experimental data and graphical representations in AP Biology.
  3. Synthesize information from cell biology, genetics, and biochemistry topics.
  4. Apply critical thinking skills to simulate exam conditions and enhance test readiness.

AP Biology Unit 3 Review: FRQ, MCQ & Test Cheat Sheet

  1. First Law of Thermodynamics - Energy can't be created or destroyed, only transformed into different forms, like light to chemical energy in photosynthesis. This law is the backbone of cellular energetics and explains how cells maintain their power supply. Embrace this concept to understand why your body's energy budget must always balance! IITian Academy Cellular Energetics Notes
  2. Gibbs Free Energy (ΔG) - ΔG tells you if a reaction runs on its own: a negative ΔG means it's spontaneous, while a positive one needs an energy boost. This handy metric lets you predict whether your favorite reactions will fire up or fizzle out. Mastering ΔG is like having a cheat code for reaction spontaneity! IITian Academy Cellular Energetics Notes
  3. Enzyme Catalysis - Enzymes work as biological catalysts by lowering the activation energy barrier, so reactions zip along faster without the enzyme being used up. Think of them as molecular matchmakers, bringing reactants together in just the right way. Knowing how enzymes speed things up is key to understanding everything from digestion to DNA replication! Knowunity AP Bio Unit 3 Notes
  4. Factors Affecting Enzyme Activity - Temperature, pH, and substrate levels can make or break enzyme function, and push them into denaturation if things go too extreme. Imagine Goldilocks testing porridge - enzymes need just the right conditions to stay "just right." Understanding these factors helps you predict when enzymes will be superheroes or sit out the game! Knowunity AP Bio Unit 3 Notes
  5. Competitive vs. Non‑Competitive Inhibition - Competitive inhibitors block the active site like a party crasher, while non-competitive inhibitors bind elsewhere and change the enzyme's shape. Both strategies slow down reaction rates, but in different ways. Knowing the difference is like spotting the subtle tactics villains use in a superhero movie! Knowunity AP Bio Unit 3 Notes
  6. ATP: The Energy Currency - ATP packs a punch by storing energy in its high‑energy phosphate bonds and releasing it upon hydrolysis to ADP and inorganic phosphate. It's like the dollar bill of the cell's economy - spend one ATP to power pumps, motors, and metabolic machines. Get comfortable with ATP, and you'll see why it's the universal "coin" for life's transactions! IITian Academy Cellular Energetics Notes
  7. Cellular Respiration Stages - Glycolysis, the citric acid cycle, and oxidative phosphorylation work in concert to harvest energy from glucose. Each stage yields ATP and NADH, with the big payoff coming in the electron transport chain. Visualize it as a three-act play where each act builds suspense until the final energy-rich climax! Quizlet AP Bio Unit 3 Flashcards
  8. Photosynthesis Equation & Reactions - The overall reaction (6 CO₂ + 6 H₂O → C₆H₝₂O₆ + 6 O₂) splits into light-dependent and light-independent (Calvin cycle) reactions. Light reactions capture solar energy to make ATP and NADPH, while the Calvin cycle uses them to build sugars. Memorizing this equation is like unlocking the cheat sheet for how plants power up! Quizlet AP Bio Unit 3 Flashcards
  9. Electron Transport Chain (ETC) - The ETC shuttles electrons through protein complexes in the inner mitochondrial membrane, pumping protons to form a gradient. This proton-motive force drives ATP synthase like wind powering a turbine. Understanding the ETC is critical to seeing how cells crank out the most ATP per glucose molecule! IITian Academy Cellular Energetics Notes
  10. Fermentation in Anaerobic Conditions - When oxygen runs low, cells switch to fermentation to regenerate NAD❺ and keep glycolysis rolling, though it nets far fewer ATP. It's like switching to reserve fuel - it gets you by but isn't as efficient. Recognizing fermentation's role helps you appreciate how cells adapt to stressful, oxygen-poor environments! IITian Academy Cellular Energetics Notes
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